Nuclear and cytoplasmic localization of interferon-tau in in vitro-produced bovine blastocysts.
نویسندگان
چکیده
Experiments were conducted to detect interferon-tau in bovine in vitro-derived blastocysts by transmission electron (TEM) and confocal microscopy. TEM showed the presence of IFN-tau in the cytoplasm and the nuclei of expanded blastocysts. Confocal microscopy similarly confirmed the presence of IFN-tau in the trophectoderm of blastocysts. The distribution of IFN-tau appeared variable with some cells showing strong labeling while others appeared to be devoid of the protein.
منابع مشابه
O-11: Diverse Effects of Polyunsaturated Fatty Acids on Oocyte Maturation and Development In vitro
Background: Polyunsaturated fatty acids (PUFAs) have been shown to influence fertility and endocrinology of reproduction and metabolic activity in many species. In dairy cows, we and others have shown changes in steroid and metabolic hormones and prostaglandins leading to alteration of ovarian activity and uterine function. These can influence fertility by changes in folliculogenesis cyclicity ...
متن کاملThe effects of activated-omental extract on nuclear and cytoplasmic in vitro maturation of rat oocytes
Objective: The role of growth factors, including vascular endothelial growth factor of activated omentum on mitosis is clearly known, though not on all the aspects of in vitro oocyte maturation. This study was designed to assess the effect of activated-omental extract (AOE) on in vitro maturation (IVM) of rat cumulus-oocyte complexes (COCs). Materials and Methods: In this experimental study, th...
متن کاملSuccessful cryopreservation of in vitro derived bovine blastocysts in microcapillary pipette tips
The open pulled straw has been used effectively to vitrify preimplantation embryos because of geometricfeatures that allow rapid rates of temperature exchange. One possible inexpensive alternative to the openpulled straw are commercially-available microcapillary pipette tips commonly used for electrophoresis. Themain purpose of this study was to compare the survival rates of in vitro produced b...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Reproduction, nutrition, development
دوره 46 1 شماره
صفحات -
تاریخ انتشار 2006